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What’s in the Fridge?”: Collections of Invasive Insect Pests from Around the World


What’s in the Fridge?”: Collections of Invasive Insect Pests from Around the World

By Mark Hoddle | January 10, 2014

Anna Kuchment, Editor for Scientific American, produced a very nice article (“End of Orange Juice ” [Scientific American vol. 308 pp. 52-59, March 2013]) on the Asian citrus psyllid and huanglongbing situation in California, Florida, and other countries affected with this pest and disease.

During Anna’s visit to UC Riverside to cover this story, we pulled out insect specimens of other pests we are working on including the red palm weevil, gold spotted oak borer, and other potential invasive pests like the avocado seed moth, Stenoma catenifer . These collections, along with other insects like the big rhinoceros beetles that attack palms in Asia, interested Anna, and this led to her developing the “What’s in the Fridge?” series for Scientific American.

The inaugural release of “What’s in the Fridge? ” occurred in the July 2013 issue of Scientific American (vol 309 [1], pp. 18). Four insects are featured, the rhinoceros beetle that was collected in Sumatra Indonesia attacking newly planted oil palms. The coconut palm weevil, Rhynchophorus vulneratus , which has invaded Laguna Beach in southern California, but has not been detected for over 18 months despite trapping efforts. This species was synonomized with R. ferrugineus , the red palm weevil, but emerging results from molecular studies clearly show that R. vulneratus is a species distinct to R. ferrugineus, and the synomization of these two species was incorrect, hence we refer to this weevil as R. vulneratus. The gold spotted oak borer , an invasive pest native to southern Arizona, has killed > 22,000 native oak trees in southern California, and recent oak mortality estimates by Tom Scott , suggest that mortality may now be as high as 80,000 oak trees in affected areas in San Diego County. Avocado seed moth is a pest that is not yet in the USA, but increasing imports of fresh avocados from countries in Latin America where this pest is native may increase the chances of this pest accidentally invading and establishing in California, Florida, and Hawaii, states where avocados are grown commercially.

Claudia, Mark and Spencer examine insects for the shoot.

Claudia, Mark and Spencer examine insects for the shoot.

The photos for the “What’s in the Fridge?” piece were taken by Spencer Lowell who was assisted by Claudia Lucia . These guys made our disorganized lab freezer look amazing!

 

Topics: Invasive Species | 1 Comment »

Entomophagy: Farming Palm Weevils for Food

By Mark Hoddle | September 30, 2013

 

Article by Mark S. Hoddle, Department of Entomology, University of California, Riverside CA 92521, USA

Background: Entomophagy is the consumption of insects by humans for food. This is an ancient practice that tends to be concentrated in certain parts of the world, notably tropical and sub-tropical regions, where there is a diversity of large insects that are suitable for eating and which also have long windows of availability, this may sometimes be year round for certain species. Insects are touted by enthusiasts and some NGO’s and global humanitarian organizations (e.g., FAO) as readily available super foods because they are excellent low cost sources of protein and essential nutrients. Edible insects can often be sustainably harvested from wilderness areas and have very low carbon footprints if farmed for personal consumption or as a commercial enterprise. Due to interest in eating insects because of their nutritional and environmental benefits, and given the fact that a wide diversity of insects, probably many tens of species across several orders (e.g., Coleoptera [beetle larvae and pupae], Hemiptera [ giant water bugs and cicadas], Hymenoptera [ants and bees], Isoptera [termites], Lepidoptera [moth and butterfly larvae and pupae], and Orthoptera [cricket, grasshoppers, and locusts]), are eaten by daily by millions of humans, there is interest in the viability of mass collection, production, or farming of certain species for food. This is already being done in some areas, with a notable example being the mass collection and subsequent preparation and sale of chapalines, a type of grasshopper, in Oaxaca, southern Mexico. Another insect that has significant potential for mass production or farming are palm weevils. Larvae and pupae of these species are widely eaten in southeast Asia and farmed to a limited extent in some countries, notably Thailand.

The Hult Prize and the Clinton Global Initiative: On Monday 23 September in New York City, the Clinton Global Initiative awarded the Hult Prize worth $1 million(US) to a team of MBS student entrepreneurs from the Desautels Faculty of Management at McGill University in Montreal Canada. The winning pitch made by this team was to combat hunger and nutrition deficiencies in impoverished regions by improving diets with insect-based meals. As part of the development McGill’s entomophagy project, Gabriel Mott (McGill) together with Mark Hoddle (UCR) traveled to Thailand together over 14-19 September 2013 and with the help of Nittaya Ummarat, a post-graduate researcher at the UC Kearney Agricultural Research Center, assessed red palm weevil farming practices for possible translation to west Africa where palm weevil are eaten, but they are not farmed.

KCRW 89.9: Mark Hoddle talks with Evan Kleiman, host of Good Food, about the Clinton Global Initiative’s award. [Interview starts at 12 minutes] Listen  Download

(A) Nittaya Ummarat and Mark Hoddle interview a red palm weevil farmer near Trang in Thailand. (B) Eating freshly stir fried red palm weevil in Thailand. (C) Gabriel Mott examining adult red palm weevils at a weevil farm in Thailand.

Top Left:  Nittaya Ummarat and Mark Hoddle interview a red palm weevil farmer near Trang in Thailand. Bottom Left: Eating freshly stir fried red palm weevil in Thailand. Right: Gabriel Mott examining adult red palm weevils at a weevil farm in Thailand.

Adult Red Palm Weevil

Adult Red Palm Weevil

Palm Weevil Biology – An Overview: Palm weevils, Rhynchophorus spp. (Coleoptera: Curculionidae), are equatorial in distribution and there are approximately 10 described species. Rhynchophorus weevils are remarkably similar in their biology and ecology regardless of what country or region they are native too. First, the immature stages all feed internally on palms. Feeding activity is typically concentrated in apical regions (i.e., palm crowns of coconuts) where meristematic tissue is present and then feeding larvae burrow down into the top portion of the trunk (an exception to this are attacks on date palm trunks where the majority of attacks occur within 1 m of the ground). This feeding strategy invariably kills infested palms, and in many regions (native and non-native ranges) palm weevils are considered pests when they attack and kill palms of economic (i.e., human food sources) or aesthetic (i.e., ornamentals in urban areas) value. This feeding pattern is typical of R. ferrugineus, the red palm weevil, and R. palmarum, the South American palm weevil, both of which are well recognized pests of coconut palms, and in areas where they are invasive, similar destruction of exotic ornamental Canary Islands palms is observed. Second, all Rhynchophorus weevils have large larvae that feed internally inside palm trunks. The entire life cycle of immature stages is concealed – larvae feed inside palms and pupae are protected within fibrous and extremely tough cocoons that occasionally fall to the ground from infested palm trees. Third, adult weevils, are large, long-lived (several months), and very vagile. Adult palm weevils release aggregation pheromones that attract males and females of the same species to palms that are undergoing attack. Consequently, palm attack intensifies over time due to recruitment of reproductively active adults looking for mates and egg laying sites and development of increasing larval populations that result from females laying eggs. Infested palms release airborne volatiles that when combined with aggregation pheromones, act synergistically in recruiting adult palm weevils.

The life cycle of palm weevils is simple. Female weevils lay eggs inside holes that they make in suitable areas (e.g., bases of palm fronds where they attach to the palm crown) with their long snout or rostrum. Weevil larvae hatch from eggs and burrow into the palm where they commence feeding. Larvae pass through several developmental stages, the exact number seems to vary and may be related to food quality, and probably human uncertainty in accurately classifying larval life stages. As larvae feed, they turn palm material into a fermenting mash. The odor of this material is very obvious and has a characteristic signature once you are aware of it. It is likely that there are important symbiotic relationships between a variety of bacteria that are associated with fermenting palm material and weevils, and these microbes may be necessary for weevil larvae to exploit palm material. Additionally, it is possible that palm weevils have evolved special adaptations that allow them to live in this extremely warm and damp environment yet somehow escape organisms that cause disease epidemics. There appear to be no reported epizootics killing high densities of weevil larvae living inside palm trees (it is possible these have been overlooked because they occur inside palms and would be very difficult to observe, but lots of dead larvae and pupae would be obvious if detected and considerable work on several palm weevil species has been conducted in the field). Once larval development is complete, pre-pupal larvae spin tightly woven cocoons from palm fibers within which they pupate. Cocoon spinning requires larvae to be wedged into a substrate upon which sufficient purchase can be attained to allow the larva to simultaneously rotate and spin palm fibers into a cylindrical, cigar shaped cocoon within which they will pupate. Upon completion of pupation, adult weevils emerge and they may breed within the palm host they occupy or they could disperse to new areas. When suitable host palms are nearby weevil flight appears to be limited in distance and attacks on neighboring palms are more likely to occur and attacks are thus aggregated in distribution. In the absence of near neighbors, weevils can fly significant distances, > 20 km in a 24 hr period, to find host palms. When suitable breeding sites are present adult weevils are reluctant to leave and they exhibit high levels of thigmotaxis, that is, they have a strong behavioral trait to secret themselves into concealed and difficult to access protective crevices in palms. Dislodging adults from these hiding sites is extraordinarily difficult and can sometimes result in physical damage to adults.

Palm weevil larvae, pupae, and adults are used for food in many equatorial countries where these weevils are native, most notably in southeast Asia (e.g., Thailand, Indonesia, Malaysia, and Papua New Guinea) and parts of South America. Palm weevil consumption is advanced in Thailand with mass production of larvae and pupae for eating, and adults being sold to initiate colonies by other farmers.

Red Palm Weevil Farming in Thailand: Over the period 14-19 September 2013, red palm weevil (RPW) farming around Trang in southern Thailand was studied to better understand production practices. Three different farms were visited and two different farming techniques were observed: (1) ground palm material contained in plastic bins (2 farms) and (2) use of rounds of palm trunks (1 farm) for rearing RPW.

Containerized Production of Red Palm Weevils

Left: Palm material being ground up to make weevil mash. Center: Palm mash being soaked in water for 3 days prior to being inoculated with adult weevils that lay eggs in the mash. Right: Palm weevil larvae growing in mash. The palm bark slices have been lifted up to expose the larvae.

Containerized Production of Red Palm Weevils: RPW can be readily grown from eggs to adults in large round plastic containers that would normally be used as basins for holding water or animal feed. Palm material, either mechanically shredded central portions of sago palm trunks (the outer bark is sliced off and used to cover the palm mash in basins [see below for more details]) or the shredded rachis of coconut palm fronds (the leaflets are sliced off with a machete and removed, as is much of the outer green epidermis covering the rachis). Coarse chips of palm material and slices of palm trunk bark are soaked liberally in water in basins. After 3 days, water is drained, the mash is hand squeezed to remove excess water and approximately 500 g of pelleted pig food (made of rice husks, corn, soy bean, sunflower seeds, and peanuts) is added to the palm mash and stirred in. Adult weevils either collected from infested palms in the wild or reared previously are used to inoculate containers with palm mash. Three male-female pairs are introduced per container and removed after 15 days. Adults are then combined to make groups of 5 pairs and used to inoculate new containers. Adults may be discarded after two rounds of container inoculation. Once adults are added to containers, the mash is covered with the slices of water-soaked palm trunk bark that was removed prior to grinding the heart of the sago palm trunk. These bark slices provide protective cover for adult weevils so they don’t fly away and later they provide the substrate and long palm fibers needed for cocoon spinning by mature larvae.

Economic Returns from Containerized Rearing: After approximately four weeks (harvest may begin as early as 17-20 days post inoculation with adults) larvae are ready to be harvested and each ~30 liter container can yield up to 2 kg of larvae (1 kg = approximately 150 large weevil larvae). All life stages of the weevil are utilized: Larvae are sold for 250 Thai Bhat per kg ($1US = 32BHT), pupae = 400BHT/kg, adults are sold individually for 3BHT.

RPW rearing is a low cost enterprise in terms of supplies and labor, and production supplements other income streams. One farmer (a rubber farmer in Songkhla Province; other weevils farmers main income streams were from rice and oil palm production or in one case the rubber plantation had been converted to oil palms and weevil farming was the family’s only income until oil palms began production) estimated that 12 rearing containers cost 460BHT, 1 kg of pig food amendment cost 32BHT, sago palm was harvested locally for free (one farmer in Nakhom Si Thammarat Province who grew RPW to sell adults to other farmers bought rounds of sago palm trunk at a cost of 1000BHT for 25 pieces each being 0.5 m tall), and just a few hours of work per week were needed to manage the rearing program.

At the weevil farm in Songkhla Province, containers of RPW larvae were stacked on shelves in a cinder block storage shed (the shed lacked temperature, humidity, and light control). The storage space, approximately 30 m2, held 130 bins stacked vertically in columns of 2-3 containers on shelves. A rough estimate based on the data above suggests that if each bin produced 2 kg of RPW larvae at 250BHT/kg, this would yield 260 kg/mo and 65,000BHT (or about $2,000US assuming all larvae are sold), which equates to a return of about $24US/m2 of rearing space. The production statistics could not be verified during this field trip, but the price per kg of larvae cited by weevil farmers was consistent at around 250BHT/kg. Mass production of RPW would appear to very profitable, but realized returns may be lower than estimated here due to variable demand. A second farmer in Nakhom Si Thammarat Province, indicated that daily sales could very variable, ranging from 0-1 kg sold per day up to 10+ kg/day if religious festivals and holidays were being observed. Infrastructure costs to house rearing containers may actually be very low too.

Low Cost Rearing Structures of Red Palm Weevil

Left: Red palm weevil rearing shed in Thailand, only 1/3 of this shed was used for rearing larvae. Center: Shelves with weevil larvae rearing containers in the mass production shed. Right: The harvest from the rearing containers, approximately 2 kg of weevil larvae.

Low Cost Rearing Structures: A rearing shed is probably not needed as long as containers are protected from rain to stop them accumulating excessive water from rain events. A tarpaulin tied between trees may be sufficient to provide shelter from excessive rain and sun events and will still allow adequate air flow over containers. The mash in which larvae are grown is highly saturated and modest rain accumulation would likely be tolerable. Year round ambient temperatures in tropical areas, like south Thailand, are likely suitable for weevil farming, and low temperature events, should they occur, may in part be significantly offset by heat given off by fermenting palm mash in which weevil larvae are developing.

Palm Trunk Rearing: One weevil farming operation in Nakhom Si Thammarat Province used rounds of fan palm trunks (Corypha umbraculifera) approximately 0.33-0.5 m tall for rearing. Rounds were purchased at 120BHT each. Individual rounds were placed under crudely built shade structures and on top of each round fermenting palm mash was placed to a depth of about 2-3 cm. Adult RPW were placed on the mash and then covered with flaps of palm bark that was pushed firmly into the mash thereby providing a protected space for the adults live, feed, and lay eggs. No preparation of palm trunks other than the application of palm mash and bark pieces is needed for this rearing set up. After approximately 3 months the first crop of weevils is ready to be harvested, and each palm round can produce larvae for up to 6 months for a total production of about 3 kg of larvae. This farmer preferred the flavor and texture of weevil larvae and pupae produced from fan palm trunks and weevil development is slower and palm trunk longevity is greater when compared to a similar set up with sago palm trunk sections.

Red Palm Weevil Rearing in Palm Trunks

Left: Cut palm logs to be used for rearing palm weevils. Right: Palm logs inoculated with palm weevils in Thailand.

Cost Comparisons of Weevil Larvae to Other Protein Sources: Weevil larvae are sold for 250BHT/kg and pupae retail for 400BHT/kg. To determine price competitiveness, the per kg prices for other protein sources being sold in nearby super markets was determined: fish 69-250 BHT/kg (price was dependent of fish species), chicken breast (with skin and bone or skinless-boneless) 128-180 BHT/kg, and pork (cured) 215BHT/kg. In comparison to these protein sources, locally-grown weevil larvae and pupae are expensive. Interestingly, one customer in Nakhom Si Thammarat Province who was purchasing 2 kg of weevil larvae to feed 5 people said the product was good value for money, affordable, and was a staple monthly dinner item.

Preparation of Weevil Larvae and Pupae for Eating: There are three main ways RPW larvae and pupae are prepared for eating: (1) stir-fried in a wok (very common), (2) prepared as a curry dish with vegetables, or (3) battered and deep fried. Sometimes live larvae may be eaten after floating in soy sauce. Cooked RPW larvae and pupae provide a substantial and hearty meal either on their own or when supplemented with additional vegetables and rice or noodles.

To prepare weevil larvae for cooking, larvae and pupae are soaked for approximately 10 min in a ~10% brine solution. Larvae are drained and blanched for 1 min in boiling water after which the head capsules of larvae may be removed prior to cooking. Larvae are added to a hot wok with vegetable oil and stir fried with Thai basil, finely chopped hot chili peppers, salt, black pepper, and soy sauce. Larvae are cooked until they start to turn a light brown in places (approximately 5 mins). This preparation may be eaten as a finger food snack with cold beer or as a main course with rice. The head capsules of stir fried larvae are crunchy, similar to small sunflower seeds, and add texture to the mouthful. RPW pupae are exquisite, there is no crunchy head capsule, the fat content is high, and the texture and consistency is similar to butter. Deep fried larvae are excellent, and without the head capsule the consumer would be unaware that the morsel was an insect larva and the dish could be easily passed off as calamari or some type of sea food.

Stir Fried Red Palm Weevils

Stir fried red palm weevil larvae prepared directly at a local weevil farm in Thailand.

Product Added Value: In addition to producing food, palm weevil farming has several very useful by-products that can be sold. First, the mash used to rear weevils can be sold as compost to amend soils. Second, the spent mash if set up in special storage areas can be left to drain and this “water” can be collected and sold as fertilizer, similar to worm water fertilizer that is collected from worm composting facilities. Third, in the case of using palm logs for rearing, the hollowed out palm trunks can be cleaned up and sold as containers for growing plants.

Summary: Weevil larvae are very easy to farm, production costs are low, and profit margins are potentially high. There is uncertainty concerning the consistency of market demand in some areas, this seemed to be the case in rural areas in Thailand where these field surveys were conducted, and where weevil production is concentrated. Sales can fluctuate and may be driven, in part, by significant religious or holiday events. Palm weevils are well suited to artificial containerized rearing. This is probably not surprising since the container in which weevil larvae are reared is functionally equivalent to a palm trunk within which weevils would normally breed. Preparation of weevils, especially larvae, for cooking is straight-forward and because larvae lack legs and antennae for example, it is easy to “disguise” the fact that they are insects when prepared for consumption. This is especially true when larvae are battered and deep fried. Cooking weevil larvae and pupae in creative ways, and perhaps developing a catchy marketing name, may enhance marketability and acceptance as a food, especially in areas where entomophagy is uncommon or insects are viewed as unclean and unsuitable for eating.

Background Reading on Entomophagy and Red Palm Weevils

  • Dembilio, O., Jacas, J.A. 2011. Basic bio-ecological parameters of the invasive red palm weevil, Rhynchophorus ferrugineus (Coleoptera: Curculionidae), in Phoenix canariensis under Mediterranean climate. Bull. Entomol. Res. 101, 153-163.
  • Dembilio, O., Tapia, G.V., Téllez, M.M., and Jacas, J.A. 2012. Lower temperature thresholds for oviposition and egg hatching for the red palm weevil, Rhynchophorus ferrugineus (Coleoptera: Curculionidae), in a Mediterranean climate. Bull. Entomol. Res. 102, 97-102.
  • Edible Insects: Future Prospects for Food and Feed Security available here.
  • Faleiro, J.R. 2006. A review of the issues and management of the red palm weevil Rhynchophorus ferrugineus (Coleoptera: Rhynchophoridae) in coconut and date palm during the last one hundred years. Int. J. Trop. Insect Sci. 26, 135-150.
  • Murphy, S.T., Briscoe, B.R. 1999. The red palm weevil as an alien invasive: biology and the prospects for biological control as a component of IPM. Biocontrol News and Inform. 20, 35N-46N.

 

Crickets vs. Palm Weevils for Mass Production for Human Consumption: Several advantages exist for palm weevil mass production over crickets. First, relatively few weevil larvae are needed to provide a very hearty meal and the texture is very agreeable, this increases greatly if pupae are eaten. Second, weevil larvae can prepared in a variety of different ways for consumption (e.g., deep or stir fried, curries, or even raw), and in comparison, preparation options for crickets may be more limited (unless they are ground into flour which is then used for cooking). Third, weevil larvae are more amenable to being “disguised” in food as they have no legs and antennae, and heads can be easily cut of prior to cooking. Fourth, immature weevils and adults are very docile and easy to collect, hold, and transfer amongst rearing containers. In comparison all stages of crickets (except eggs) have the capacity to jump, walk, and fly (when adults). This makes day-to-day handling and management of crickets more difficult in comparison to weevil larvae. Fifth, weevil larvae are well adapted to mass production in containers, this is basically their life style in a palm trunk. Crickets on the other hand are much more mobile and free ranging in nature and typically don’t live year round in highly aggregated communities. This may limit production capacity and ease of rearing. Sixth, consequently, high density production is unnatural for crickets and may predispose them to the diseases that can wipe out high density populations in nature. Such disease or epizootic phenomena, if they exist, are not known for palm weevils.

Topics: Entomophagy, Red Palm Weevil | 6 Comments »

Pheromone Trapping Program for the Brown Marmorated Stinkbug

By Mark Hoddle | June 17, 2013

A Pheromone Trapping Program for the Brown Marmorated Stinkbug (Halyomorpha halys) Begins in Los Angeles County, California

Brown marmorated stinkbug (BMSB), Halyomorpha halys (Hemitpera: Pentatomidae), is an invasive insect pest native to China, Japan, Korea, and Taiwan. It was first discovered and officially documented on the east coast of the USA around 1998. This pest has also been reported from numerous US states including: California, Connecticut, Delaware, Indiana, Kansas, Kentucky, Maine, Maryland, Massachusetts, Mississippi, New Hampshire, New Jersey, New York, North Carolina, Ohio, Oregon, Pennsylvania, Rhode Island, South Carolina, Tennessee, Virginia, Washington, D.C. and West Virginia. It may also have established populations in Switzerland and Canada.

A Brown Marmorated Stinkbug pheromone trap set up in Pasadena

A Brown Marmorated Stinkbug pheromone trap set up in Pasadena

This stinkbug has a very broad host range having been recorded feeding on tree fruits, vegetables, shade trees, and legume crops, with a strong preference for apples, plums, pears, peaches, and cherries. It has caused significant economic damage to agricultural crops on the east coast, especially apples. In 2010, this pest replaced key apple pests, such as codling moth, as the primary pest attacking apples, and it was estimated that the economic impact to the apple industry on the east coast of the USA could have exceeded $37 million. Feeding damage results when immature BMSB (called nymphs) and adults puncture fruit with their piercing-sucking mouthparts. This feeding damage causes distortion of developing fruit and is referred to as “cat-facing.”

Brown marmorated stinkbug is a “true” bug, meaning it has piercing-sucking mouthparts (as opposed to chewing mandibles like you would find on a caterpillar or beetle) and it has incomplete metamorphosis whereby nymphs (the immature stages that develop following egg hatch) gradually adopt the adult shape and color each time they molt into the next developmental stage. Butterflies, moths, flies, and beetles, for example, have complete metamorphosis, whereby a larva develops into a pupa (a cocoon or chrysalis) and then emerges as an adult that doesn’t resemble the larva. Brown marmorated stink bug has six immature stages, the egg, and five nymphal stages, before reaching the adult form.

Management programs for this pest are being developed on the east coast and include; (1) biological control agents, (2) pesticide evaluations, and (3) pheromone traps to monitor the presence, abundance, and seasonal phenology of this pest. The biological control program is being developed by the USDA-ARS and is focusing on the use of egg parasitoids sourced from China, part of the pest’s native range. Surveys on the east coast have revealed that native stinkbug parasitoids are not effective at attacking BMSB eggs. This escape from natural enemies may be one potential reason why BMSB has proliferated in the USA.

In addition to damaging crops, BMSB forms very dense overwintering aggregations inside houses, sheds, and garages. Because these bugs have “stink glands” on the dorsal (top side) surface of the abdomen and ventral (bottom side) of the thorax this gives them an unpleasant characteristic odor, especially when disturbed. Consequently, having large numbers entering dwellings and a need to remove them physically can result in high levels of irritation for homeowners.

Detections of BMSB in California have occurred since at least 2005. Finds have occurred from Costa Mesa in Orange County in southern California north to areas around San Francisco. Detections have been made on transport and storage containers, vehicles, plants, boats, and firewood being shipped from the east coast to California. BMSB appears to be able to hitchhike considerable distances on these inedible structures. It is possible there are now breeding populations in and around Los Angeles County, especially the Pasadena area, where there have been reports of large overwintering aggregations forming inside garages.

A Brown Marmorated Stinkbug Crew with a trap at Huntington Gardens in Southern California

A Brown Marmorated Stinkbug Crew with a trap at Huntington Gardens in Southern California

In response to this emerging threat, the University of California Riverside, UC Cooperative Extension, and the California Department of Food and Agriculture have set up a cooperative project with the USDA-ARS to test pheromone traps to monitor for BMSB in Los Angeles County, and additional areas in northern California (e.g., around Stockton in San Joaquin County).

In southern California, trapping programs are being run in cooperation with the LA County Agricultural Commissioner’s office (Gevork Arakelian, the LA County Entomologist), private home owners in Pasadena, the Huntington Gardens and the LA Arboretum.

The trapping program is evaluating different types of lures that BMSB may find attractive in southern California. Identical trials are being run simultaneously over the summer (2013) on the east coast. The goal is to determine which lures are most effective at attracting BMSB and whether these results are consistent between the east and west coasts of the USA. The development of a sensitive and species specific monitoring tool will be very valuable for California as it will allow the detection of small populations before they become abundant and troublesome. Traps will also allow a more precise delineation of areas infested with BMSB in California without having to wait for homeowner reports or a need to undertake difficult and time consuming visual surveys of plants in areas suspected to be infected with BMSB. The next phase of the BMSB program for California will be to begin working with the USDA-ARS on developing a biological control program with egg parasitoids. Starting this program early, before BMSB becomes a severe problem similar to the east coast, could help greatly to alleviate economic losses and minimize the annoyances associated with overwintering aggregations inside houses.

Topics: Brown Marmorated Stinkbug, Invasive Species, Mark Hoddle | 12 Comments »

Searching for natural enemies of the goldspotted oak borer in Arizona

By CISR Team | May 29, 2013

Vanessa Lopez

Vanessa Lopez
University of California, Riverside

 

Written by:
Vanessa Lopez (Ph.D. Candidate, UC Riverside)
Mark Hoddle, Ph.D. (Biological Control Specialist and Principal Investigator)

Photos by:
Mike Lewis and Vanessa Lopez

 

 

Background

The goldspotted oak borer (GSOB), (Agrilus auroguttatus) (Coleoptera: Buprestidae), is a recent pest of native oak trees in southern California.  This fairly small beetle (about 10 mm long and 2 mm wide) is native to the mountains of southern Arizona, and was likely introduced into the Cleveland National Forest in San Diego County, California through the unintentional movement of infested oak firewood.  GSOB was first collected in San Diego County in 2004, but was probably introduced into the area several years earlier.  This beetle has killed more than 22,000 native California oaks in a relatively short time period, and continues to spread into new areas within southern California.  In fall 2012, GSOB was found in Idyllwild, Riverside County, about 80 miles from the GSOB infestation epicenter, This find resulted in immediate eradication efforts by local government agencies including CAL Fire and the US Forest Service.  GSOB was also detected in 2010 in Marion Bear Memorial Park, San Diego County, about 65 miles from the GSOB epicenter and has likely established here. Movement of infested oak firewood out of the Cleveland National Forest probably moved GSOB into these new areas.

In southern California, GSOB aggressively attacks larger (more than 12 cm in diameter) coast live oak (Quercus agrifolia), California black oak (Q. kelloggii), and canyon live oak (Q. chrysolepis), and can even attack and kill healthy trees.  Trees infested with GSOB die from repeated larval feeding damage to the cambial layer, which is the water and nutrient transportation system of the tree.  Although adult GSOB feed on oak foliage, adult feeding does not result in tree injury.

In southern Arizona, where this beetle is native, GSOB is not a pest.  In its native habitat, this beetle infests stressed tress, and can actually be very difficult to find.  Since GSOB occurs at much lower population levels in Arizona, there may be natural enemies that have evolved to efficiently find and consume these beetles.

Developing a classical biological control program for GSOB in California

Since 2009, research towards the development of a classical biological control program for GSOB in California has been ongoing.  The goal of classical biological control is to restore balance to an ecosystem by reuniting an invasive pest with its host specific co-evolved natural enemies.  To find natural enemies that have co-evolved with and only attack GSOB, we’ve conducted several surveys throughout the home range of this beetle, and are continuing our search in summer, 2013.

Searching for egg parasitoids of GSOB

Surveys for egg parasitoids of GSOB were initiated in summer 2012 using field deployed GSOB egg masses to attract and potentially become parasitized by these tiny wasps.  For eight weeks, GSOB egg masses were set out and collected every seven days in the Santa Rita Mountains, Pima County, Arizona and in the Cleveland National Forest, San Diego County, California.  Surveys in Arizona resulted in the collection of the first known egg parasitoid of GSOB, a Trichogramma species that is a currently undescribed generalist parasitoid wasp which likely attacks eggs of a lot of different species.  No egg parasitoids were found in California.  Additional GSOB egg parasitoids surveys are planned for this summer (2013) in Arizona.

Continuing surveys in summer 2013

GSOB has been collected from several mountain ranges across southern Arizona including the Chiricahua, Dragoon, Huachuca, Santa Catalina, and Santa Rita Mountains.  To find field sites for the 2013 GSOB egg parasitoid surveys, we took a road trip through southern Arizona to search for active populations of GSOB.  GSOB densities in Arizona are naturally very low, so this beetle can be difficult to find in its native range.  Trees showing symptoms of decline (crown die-back, patches of pale and dry leaves, and bark staining) were ground checked for evidence of GSOB infestation (D-shaped exit holes, larval feeding galleries, and GSOB life stages), but the majority of death or decline in the oaks we encountered was due to fire damage.  However, we did encounter several sites with GSOB activity in the Chiricahua, Dragoon, Huachuca, and Santa Rita Mountains which we will use in summer 2013 to search for GSOB egg parasitoids by hanging GSOB eggs onto infested oak trees.

Ultimately, if we can find a host specific egg parasitoid of GSOB eggs it may be a candidate for eventual release in California to help control this invasive pest. So far our surveys suggest that GSOB eggs in California are not attacked by parasitoids and this could be one reason why populations of this pest are so high in the Cleveland National Forest when compared to forests in Arizona. Restoring an egg parasitoid from Arizona with GSOB in California may help reduce the densities of this pest and alleviate some of the damage it causes.

 

Topics: Goldspotted Oak Borer | 1 Comment »

Has the Red Palm Weevil Gone Extinct in Laguna Beach?

By Mark Hoddle | March 28, 2013


The Situation: On 27 March 2013, a Canary Island palm on the Pacific Coast Highway that had been previously inspected and treated for red palm weevil (RPW) infestation in June 2011 was removed by MPA Landscape Services (Chuck Galanti). The palm was removed because it was causing structural problems to an adjacent building, and not because it was damaged by RPW.

The felling of this palm provided an excellent opportunity to examine the crown for evidence of RPW activity two years after the palm was treated with insecticides.

Careful inspection of every frond that was removed failed to find evidence of RPW tunneling, holes, or RPW cocoons wedged into cavities at the bases. This damage had been observed in June 2011.

Further, dissection of the meristematic bulb of the palm, the most preferred feeding site for RPW larvae, failed to detect any evidence of RPW feeding activity. No tunnels or feeding damage was observed, no larvae, pupal cocoons, or adults (in 2011 body parts of dead RPW adults were found in the crown of this tree) were found.

Has RPW Gone Extinct in Laguna Beach? The last live RPW to be captured in Laguna Beach was January 2012 when a single adult was trapped in a CDFA bucket trap baited with aggregation pheromone, ethyl acetate synergist, and fruit. Enhanced trapping trials run over June-July and October-November 2012 using stacks of cut date palm logs and pheromone baited bucket traps failed to capture RPW.

Given the lack of RPW captures and no recent signs of RPW feeding damage to Canary Island palms in Laguna Beach it is possible that RPW has either gone extinct or populations are so low as not to be noticeable.

International monitoring protocols suggest that before a pest can be considered eradicated monitoring programs must fail to detect the pest for three consecutive years.

Why Would RPW Go Extinct in Laguna Beach? There are several potential reasons why RPW populations may have gone extinct in Laguna Beach. The first impediment to successful invasion could be a lack of genetic diversity. DNA analyses of RPW collected from Laguna show very little genetic variation suggesting that the founder population was small. Consequently, inbreeding may have resulted in an unhealthy and weak population that was susceptible to environmental events that cause extinction (e.g., climate or pesticides). Second, the climate in Laguna Beach is cool, much cooler year round than the tropical climates of southeast Asia where this weevil is native. Low temperatures may not be favorable for year round RPW breeding, development, and dispersal. This would affect population growth and stability. Third, the variety of RPW in Laguna (black with a red stripe) may not perform well on Canary Island palms. Its preferred host in southeast Asia is coconut palm. There are no coconuts in Laguna Beach as it is too cool for this palm to grow well. Fourth, targeted pesticide treatments of RPW-infested palms may have been very successful at exterminating small RPW populations before they could grow large enough to spread into more favorable areas.

Are California’s Palms Safe from Palm Killing Weevils? No! There is another palm killing weevil invading southern California from Mexico. This is the South American palm weevil, Rhynchophorus palmarum. This pest has killed Canary Island palms in Tijuana and adult weevils have been trapped in San Diego County and southern Texas. It may only be a matter of time before this pest establishes in southern California and starts killing palms.

The Back Story: Red palm weevil (RPW) was first discovered in Laguna Beach in October 2010 following the inspection of a Canary Island palm that had died on a residential property. The infested palm was removed and destroyed.

RPW is native to southeast Asia and has been moved internationally through trade in live palms. This beetle is a highly destructive palm pest that has invaded many countries and killed hundreds of thousands of palm trees.

It is possible that RPW was intentionally introduced into California for food as the larvae are edible either cooked or raw.

The Response to Detecting RPW: In response to this RPW find in Laguna Beach, a red palm weevil Technical Working Group (TWG) was formed to develop a management plan for this highly destructive invasive pest.

A pheromone trapping program was initiated to delineate the infestation zone in Laguna Beach and a series of town hall style meetings were conducted in Laguna Beach and Coachella Valley by the California Department of Food and Agriculture (CDFA) to alert the public and stakeholders (e.g., date and ornamental palm producers) to this problem.

Additionally, a surveillance program was initiated and this was a joint effort between the University of California Cooperative Extension (John Kabashima [Orange County] and Mark Hoddle [UC Riverside]), the Orange County Agricultural Commissioner’s Office (Nick Nisson), and the CDFA (Mohammed Alzubaidy).

The surveillance program had two objectives: (1) to find RPW infested palms in Laguna Beach and treat them with pesticides,and (2) to run enhanced trapping trials using CDFA’s RPW pheromone traps and stacks of cut date palm logs to lure flying RPW adults to traps.

Date palm logs were acquired for these trials via cooperative efforts with Cocopah Nurseries (Duane Young) and Hadley Dates (Albert Keck) in the Coachella Valley.

This enhanced trapping trial design was motivated by RPW research that was completed in the Philippines.

Two enhanced trapping trials replicated across three different sites were conducted for four-five weeks in Laguna Beach over June-July 2012 and again in October-December 2012. These super-trapping-trials failed to capture any RPW adults (adult weevils are attracted to pheromone traps and fermenting palm logs to mate and feed and are often captured in bucket traps as a result.)

There is now mounting evidence that RPW may be extinct in Laguna Beach or populations are too low to be detected with currently available tools.

Topics: Red Palm Weevil | 1 Comment »

Update from Israel on the Polyphagous Shot Hole Borer and its Fusarium fungal symbiont

By CISR Team | March 25, 2013

Update from Israel on the Polyphagous Shot Hole Borer and its Fusarium fungal symbiont

mary_lu_arpaia_150

Mary Lu Arpaia
University of California, Riverside

Written by:
Mary Lu Arpaia (Extension Specialist, UC Riverside) David Obenland (Plant Physiologist, USDA-ARS)

Photos by:
Mary Lu Arpaia

We recently had the opportunity to visit Israel and spend several days looking at avocados including a visit with Zvi Mendel and Stanley Freeman, the lead researchers in Israel on the Polyphagous Shot Hole Borer (PSHB) and its Fusarium fungal symbiont. (The authors would like to thank them for their editorial comments on this report.)
So far the beetle is still largely confined to the central coastal region of Israel and the northern Negev. The beetle has also been found in the Upper Galilee at Kibbutz Hagoshrim in avocado and on ornamental trees in other locations in this region which is quite far from the primary infested area. The infested avocado trees have been destroyed but the beetle population already spread outside of the site of the initial infestation. Interestingly, this find was with a grower who packs their avocados in the coastal area. It is assumed that the beetles arrived in the bins originating from the infested area. This is a reminder to California growers and packers that to minimize the spread of pests clean bins are essential. The spread of avocado thrips and persea mite in California is also assumed to have been by bins containing vegetative material.

The Israeli researchers have continued searching for materials that will either control the beetle or the fungus. They have had reasonable success in the lab when they test materials under controlled conditions but application out in the field is not effective. There are no chemical treatments on the horizon that growers can use.

We visited infested avocado orchards in the Hefer Valley and the region southwest of the Carmel mountains (south of Haifa). We visited a Reed orchard which is believed to have been infested approximately 5 to 6 years. Three years ago this grove showed heavy infestation in the entire grove. What we saw on our visit was severe limb dieback, many broken branches scattered on the orchard floor, dropped mature fruit and smaller than normal fruit size for the fruit remaining on the trees. Signs of the beetle boring as evidenced by sugar exudates were easy to find wherever we looked. Dr. Mendel told us that the grower is giving up on this orchard and plans to bulldoze the orchard after harvest. We went on to see several other groves; in all except a 2-year-old orchard it was easy to find limb dieback, fallen fruit and sugar exudate up and down branches. We were told that they do not often find infestation of young groves but when they do, it is usually on the base of the trunk (either rootstock or scion).

We visited a plot with some growers along with Leo Winer (an extension officer) and Udi Gafni (head of the research and development unit of GRANOT) where insecticide applications to infested trees had been made last fall (2012). Unfortunately, signs of continued beetle activity were relatively easy to find. The growers told us about seeing fruit shriveling as the branch dies back. Since substantial fruit drop occurs of both mature and developing there is also an overall drop in productivity as an infestation spreads throughout a grove. Growers are extremely concerned and frustrated that there are no control measures for the beetle. Similar to California, Israel has historically used minimal pesticide sprays. The growers know that in areas already infested that spray applications are key to their continued orchard viability.

Avocado growers in Israel are also seeing problems with Botryosphaeria fungal infections. We visited the northern Negev Desert area where avocados are grown. We went to a large Hass orchard the grower is attempting to control this problem using phosphite injections. We were able to see upper limb dieback and staining on the upper branches.
In the meantime, Drs. Mendel and Freeman are continuing to study beetle biology and the behavior of the Fusarium fungal component. Dr. Mendel is developing a method to raise the beetle in the laboratory. This will be a important breakthrough since it will allow for a better understanding of the beetle life cycle. They know that the female beetle once it flies and seeks a place to burrow has about a 48 hour window to successfully establish itself in the host plant since this is the time period it can survive without feeding. We also learned that the beetle carries the Fusarium spores in its mycangium (a specialized structure at the back of its jaw), rather than hyphae. The larvae and pupa do not have mycangium, only the adults. While the larvae pupate, the Fusarium in the galleries sporulates and the emerging adult as it feeds picks up the Fusarium spores. On a side note, Drs. Mendel and Freeman do not necessarily agree with our use of the name PHSB. They argue that the beetle is monophagous (eats only one kind of food, Fusarium) but uses several tree species as hosts. We will have to see how the final name for the beetle is ultimately settled among the insect taxonomists.
Finally, Dr. Mendel is very worried about the spread of the beetle to native tree species in Israel, especially oaks which are found in many areas throughout the country. The box elder (which also occurs as a landscape tree in California) has been decimated by the PHSB. This is a warning flag for all California residents to take action to safeguard our native oaks, other native species and landscape trees.
These visits reinforced the extreme importance that the California industry must be diligent looking for tree infestation. The industry must work with the landscape industry and forestry service in southern California to understand how fast the infestation is spreading and what hosts are most susceptible. The industry needs to continue funding both applied (surveys, control measures and understanding of the beetle and fungal biology) as well as more basic work such as the origin of the beetle. This latter piece of information may lead to better ideas concerning control measures. Finally California researchers and industry leaders would be well advised to collaborate closely with our Israeli colleagues. They are facing this crisis head on since the PSHB has already spread into commercial groves. From their advanced experience, the California industry can learn much.

A conference on the PSHB and its Fusarium fungal symbiont was held in August 2012 in Riverside. The talks from this meeting are available for review on the website www.avocadosource.com. Video of the conference presentations from the public meeting are available for viewing on YouTube. The science portion of the meeting will be posted on YouTube in the forthcoming weeks.

Topics: Polyphagous Shot Hole Borer | No Comments »

Has the Asian Citrus Psyllid Parasitoid, Tamarixia radiata, Established in California?

By Mark Hoddle | July 19, 2012

Tamarixia radiata (female)

The Problem: Tamarixia radiata, a tiny parasitic wasp has been imported into California from the Punjab of Pakistan to attack nymphs of Asian citrus psyllid (ACP), a serious citrus pest that has established wide spread populations in the counties of Los Angeles, San Bernardino, and Riverside (significantly smaller populations are known in Imperial and San Diego Counties too). Releases of Tamarixia commenced in December 2011 after USDA-APHIS cleared this natural enemy for release from the Quarantine Facility at the University of California Riverside. Since these initial releases in December, approximately 8,500 parasitoids have been released at about 50 different sites in Southern California by July 2012.

The Biocontrol Release Program: Parasitoid releases have been made in the cities of Azusa, Bell Gardens, Chino, Duarte, Fontana, Los Angeles, Mira Loma, Montclair, Ontario, Pico Rivera, Pomona, Rialto, Riverside, San Bernardino and Whittier. In these areas, citrus in residential gardens with ACP infestations were selected for parasitoid releases. Ideally release sites had lemons or limes, and other types of citrus too (e.g., oranges or grapefruit.) Lemons and limes are good hosts for ACP because they tend to produce a lot of flush growth that is favored by ACP females for egg laying, and lemons in particular, tend to produce flush growth more frequently when compared to other types of citrus (e.g., oranges.)

A mixture of different citrus types in gardens is desirable for a release site because it increases the possibility that there will be flush present on different citrus types at different times of the year that will be available for ACP can infest. We’ve also noticed that semi-regular pruning of citrus, good fertilization, and decent watering schedules can also help garden citrus produce strong flush.

Tamarixia Recoveries: Parasitoids have been recovered at about 4-6 release sites in Azusa and Bell Gardens, and some of these sites have not received parasitoid releases for 2-3 months suggesting that Tamarixia has likely established and is breeding on its own (the life cycle of the parasitoid is about 12-14 days depending on the temperature). DNA analyses suggest that the parasitoids that have been recovered from release sites have a unique genetic signature that is very similar to the parasitoids imported from Pakistan for the biological control of ACP in southern California. This result tentatively suggests that the parasitoids recovered from release sites are most likely those that were mass reared and released by the

Tamarixia radiata parasitizing an Asian citrus psyllid nymph in Bell Gardens Los Angeles County

University of California Riverside. Further, at one site in Azusa, it appears that Tamarixia has self-dispersed about 65 m from where it was released on citrus and it established new populations on ACP infested curry plants (curry plants are really good hosts for ACP too.) Additionally, the genetic signal from captured parasitoids is reasonably diverse which suggests that the foreign exploration, rearing, and release program at UCR has preserved a lot of genetic variation which could be very important for local adaptation by parasitoids to citrus growing areas from the coast to the inland valleys.

How Does Tamarixia Kill ACP? Tamarixia can kill ACP nymphs in two different ways. First is parasitism, and in this instance a female parasitoid lays an egg underneath a fourth or fifth instar (instar refers to the developmental stage of the nymph, so a fifth instar is the fifth nymphal stage before the nymph becomes an adult) nymph. These larger nymphs are most preferred by Tamarixia for parasitism. When the egg hatches, the parasitoid larva begins to feed on the under surface of the ACP nymph. Eventually the Tamarixia larva will completely excavate the body cavity of the ACP nymph, and it will pupate inside the empty shell of its host. Often you will see beige colored silk strands radiating out from the edge of a mummified ACP nymph. The parasitoid larva spins this silk to hold the ACP shell onto to the twig that the nymph was feeding on. This ensures that the husk of the host won’t fall off the twig prematurely exposing the parasitoid pupa to predators or inclement climatic conditions. Once the parasitoid has finished pupating, the adult wasp chews a perfectly circular hole near the head of the mummified ACP husk and the parasitoid emerges. After emergence it will mate and if it is a female, it will hunt for more ACP nymphs to attack. The presence of these emergence holes near the head of dried up ACP nymphs is very strong evidence that Tamarixia emerged from that host.

The second way Tamarixia can kill ACP nymphs is by host feeding. When Tamarixia host feeds, the female uses her ovipositor or egg laying tube at the posterior end of her abdomen to stab and mutilate the ACP nymph. This physical injury causes hemolymph (the equivalent of insect blood) to leak from the body and the parasitoid feeds on this fluid. Hemolymph is an important source of protein for female parasitoids, and the trauma of being stabbed then feed upon is sufficient to kill ACP nymphs. Only females can attack ACP nymphs in this manner because males lack an ovipositor because they don’t lay eggs.
Laboratory studies on the biology of Tamarixia suggest that through the combined actions of parasitism and host feeding, individual female parasitoids have the capacity to kill several hundred ACP nymphs during their life time.

Argentine ants tending an infestation of Asian citrus psyllid nymphs. Ants may hamper biological control of ACP by Tamarixia

Ants and ACP Biocontrol: At some release sites, ants, in particular the invasive Argentine ant, may have the potential to interfere with the biological control of ACP. Field observations strongly suggest that ants tend ACP nymphs and as a reward for guarding them, the ACP provide the ants honeydew, a sweet waste product that they excrete. We’ve also seen ants capture and eat Tamarixia parasitoids foraging in clumps or patches of ACP nymphs, and in some instances the ants have chased Tamarixia off the patch if they could not catch it. It is possible that when ACP infestations are heavily tended by ants, some sort of ant control may be needed if the natural enemies are to attack the pests. This problem is not unique to ACP, honeydew producing scales and mealybugs, for example, are also tended by ants, which in turn hampers effective biological control of these pests too.

Future Plans: Monitoring and release programs are ongoing, and UC Riverside is now ramping up the mass production of Tamarixia for expanded releases throughout ACP infested zones. It is hoped that as more Pakistani Tamarixia are released in southern California greater establishment rates will occur and natural spread will begin to fill in areas between release sites.

Topics: Asian Citrus Psyllid | 2 Comments »

Testing a New Trapping Program for Red Palm Weevil in Laguna Beach California

By CISR Team | June 28, 2012


Since the first official detection of red palm weevil (RPW) in September 2010, the California Department of Food and Agriculture has been running a pheromone trapping program for this pest in Laguna Beach. As part of this trapping program, CDFA is monitoring 153 traps that are set up in two different patterns. The core area around the initial RPW finds is being trapped at a rate of 50 traps per square mile. Outside of this intensive trapping area the remaining traps are deployed at 25 per square mile. Traps were monitored weekly until March 2012, after this time, servicing has been reduced to once every two weeks. The first RPW adult caught as part of this monitoring program was collected from a trap on 18 January 2012 that was last serviced 9 January 2012. The trap that caught the single RPW was on a Washingtonia palm close to the Pacific Coast Highway in Laguna Beach.

It is surprising that despite the intensive and long-term trapping effort by the CDFA that so few RPW have been trapped. In overseas countries were RPW is either native (e.g., India) or invasive (e.g., the Mediterranean and Saudi Arabia) the pheromone traps are very effective at luring and trapping RPW. However, it should be noted that the color form of the RPW caught in these trapping programs is the orange and black form, and not the red stripe form that is present in Laguna Beach.

Orange and red stripe on the Red Palm Weevil.

There could be several reasons for the lack of RPW captures in Laguna Beach using the pheromone bucket traps:

  1. The RPW pheromone that is being used in the trapping program, while very attractive to the orange and black form RPW, is not particularly attractive to the red stripe form in Laguna Beach. May be it is not even the correct aggregation pheromone.
  2. Densities of RPW in Laguna Beach are simply too low to be captured in the bucket traps with the pheromone.
  3. The pheromone trap set up being used is not very attractive to the red stripe form of RPW in Laguna and dispersing RPW simply ignore it because they find infested palm trees more attractive and they tend to aggregate here with other RPW instead of going to the traps.

To address these potential issues a couple of research projects were executed overseas with funding from the CDFA’s Specialty Crops Program. This program was designed to investigate possible factors affecting the success of the trapping program in Laguna Beach, and if possible, to figure out ways to make it more effective.

Three key research questions were addressed with research projects that were run in the Philippines and Indonesia: (1) can the commercially-available RPW pheromone that is being used in Laguna Beach attract the red stripe form of RPW in countries where this color form is native? We had to do the work in these overseas countries because there are no other areas in the world which have the red stripe form of RPW. (2) Is there a more attractive trap set up that could increase the chances of capturing the red stripe form of RPW? (3) Does the pheromone for the red stripe form of RPW have the same chemical make up as the commercial RPW pheromone used for trapping the orange form of RPW?

Assessing the Attractiveness of the RPW Trapping Program Used in Laguna Beach to the Red Stripe Form of RPW. Two large field experiments were conducted in the Philippines and Sumatra Indonesia to assess the attractiveness of the CDFA’s bucket trap to the red stripe form of RPW. Three different trap set ups were tried: (A) cut coconut logs only (coconuts are a highly preferred host palm for RPW in countries where it is native and volatiles released from cut coconut palms are very attractive to RPW), (B) pheromone traps only, and (C) pheromone traps with freshly cut coconut logs.

The daily RPW harvest from the field trials in Indonesia

The results of these trials in the Philippines and Indonesia were crystal clear – RPW was highly attracted to stacks of cut coconut palm logs that had the pheromone traps sitting on the log stack. This suggested to us that perhaps the missing element in Laguna was a lack of freshly cut palm material to help attract RPW to the pheromone traps that the CDFA is using to monitor for this pest.

The results of this study also indicated that the commercially-available pheromone seemed to be quite important for attracting the red stripe form of RPW to stacks of cut coconut logs. But did this mean that the pheromone was actually the correct one used by the red stripe form, or did these RPW simply find it O.K., but not as attractive as the pheromone that they produce themselves (assuming the red stripe RPW have a similar but different pheromone to that used by the orange and black forms)? To answer this question we collected aggregation pheromone for red stripe RPW captured in the field in an oil palm plantation in Sumatra Indonesia.

Cut coconut palm trunks and a RPW pheromone trap set up in Indonesia to test the efficacy of the commercially-available RPW aggregation pheromone

Collecting Aggregation Pheromone from the Red Stripe Form of RPW in Sumatra Indonesia. Adult red striped RPW collected from oil palm trees that had collapsed in a commercial oil palm plantation were used for the pheromone collection experiments. The process was quite simple in concept, but a little challenging to execute in an outdoor shed in an oil plantation! Field collected RPW (all red stripe forms) were set up in oven bags that had hoses hooked up to an aquarium pump. The aquarium pump pulled air through a special set of filters to remove any potential volatile contaminants before it passed into the oven bags and over the contents of the bags. The RPW were contained in mesh cages inside the oven bags so they couldn’t chew holes in the bags and escape. Four oven bags were set up each time pheromone aeration trials were run. The bags contained either an empty sterilized mesh cage (to capture the chemical signature of the mesh cage), RPW food only in a mesh cage.

RPW set up in oven bags and being aerated for pheromone capture in Sumatra Indonesia

Sugar cane or oil palm hearts were the food used for the RPW – it really likes to eat both types, but we didn’t know whether sugar cane or oil palm would be needed to stimulate RPW to release aggregation pheromone, so we tried both. RPW in a mesh cage only, or RPW with food inside a mesh cage. Aerations were made for these different treatments continuously for about 3-4 days. The volatiles released were trapped on ultra-pure charcoal filters as purified air was pulled through the oven bags containing the treatments. After this exposure time the charcoal filters were removed, new treatments were set up again, and then randomly assigned to a new set of oven bags. The process was repeated three or four times over a 14 day period.

The RPW field crew who assisted daily with the field experiments in Indonesia

The charcoal filters with aeration product were air expressed from Sumatra to Riverside, California. Jocelyn Millar at UC Riverside analyzed the aggregation pheromone captured on the charcoal filters that had been released from the red stripe RPW inside the oven bags. Millar’s analyses clearly showed that the aggregation pheromone released by the red stripe form in Sumatra was exactly the same as the commercially-available pheromone used to trap the orange and black form of RPW. We concluded from this work that we were using the correct aggregation pheromone to attract the red stripe form of RPW in Laguna Beach.

A New Trapping Plan for the Red Striped RPW in Laguna Beach. So after the completion of these studies in the Philippines and Indonesia, the pieces were now in place to consider a modified trapping program for the red stripe RPW in Laguna. We know that the aggregation pheromone released by the red stripe RPW is exactly the same as the commercially-available pheromone used to trap the orange and black form of RPW, so there is no problem in using this pheromone. We also learned that red stripe RPW are strongly attracted to stacks of freshly cut palm logs that have aggregation pheromone sitting on top of these log stacks. The challenge after figuring this out is to replicate this set up in Laguna Beach.

Setting up the RPW pheromone aeration experiment in Sumatra Indonesia

The CDFA gave permission to set up a new trapping plan in May 2012 using freshly cut date palm logs and pheromone traps. This program was a replication of the successful trapping programs that had been tested in the Philippines and Indonesia. In June 2012, three stacks of cut date palm logs with RPW pheromone traps were deployed at three different sites in Laguna Beach. This project was a major collaboration between UC Riverside with date palm producers in the Coachella Valley, in particular Duane Young with Cocopah Nurseries in Indio California, and Albert Keck with Hadley Dates in Vista Santa Rosa (both businesses operate out of the Coachella Valley.)

Cocopah Nurseries donated 3 date palms that were felled, and from which the top most third was removed from the trunk and cut with a chainsaw into sections for deployment at Laguna Beach. These palm sections were transported to Laguna Beach and set up at three different sites with pheromone traps. These sites were selected based on their close proximity to previous find sites for RPW and to palms which have had evidence of recent RPW attacks. The log stacks and pheromone buckets are checked daily and trap checking is a cooperative effort between CDFA (Mohammed Alzubaidy), the Orange County Agricultural Commissioner’s Office (Nick Nisson), UC Cooperative Extension (John Kabashima), and UC Riverside (Mark and Christina Hoddle). At the time this blog was written, this new trapping program had been out for one week and no RPW had been caught.

Topics: Mark Hoddle, Red Palm Weevil, UC Riverside | No Comments »

Huanglongbing Detected in Hacienda Heights, Los Angeles County

By Mark Hoddle | April 13, 2012


The Situation: On Thursday April 5 2012, after about a week of testing, the California Department of Food and Agriculture (CDFA) removed a pumelo tree with a lemon graft from Hacienda Heights in Los Angeles County after the tree and an Asian citrus psyllid found on the tree both tested positive for a lethal citrus disease, Huanglongbing (pronounced Wong-Long-Bing [HLB]). HLB is caused by a bacterium, and HLB-causing bacteria kill citrus by affecting the food transportation systems of infected trees. There is no known cure for the disease, and the HLB-bacterium does not pose a threat to humans, pets, or other plants.

Asian Citrus Psyllid

The Problem: Huanglongbing, also known as yellow shoot disease, yellow dragon disease (these are English translations from the Chinese Huanglongbing indicating that the leaves of infected citrus appear an irregular mottled yellow color) or citrus greening (because fruit don’t ripen properly and remain green in areas) is spread from tree-to-tree by the Asian citrus psyllid (ACP), This small insect acts as a flying syringe carrying bacteria in its needle-like mouthparts and when infected psyllids feed on citrus they inject bacteria into trees. The disease can also spread by grafting infected branches onto healthy trees. The possibility that an infected branch was brought into California and used for a graft is being investigated.

Infected trees typically die within 5-8 years after being infected, but disease symptoms may take as long as 2 years to become obvious. This long latency period provides ample time and opportunities for ACP to visit infected plants, feed on them, acquire the bacteria and fly onto healthy trees to feed which puts these trees at risk of becoming infected with bacteria.

HLB has been a particularly devastating disease problem in Florida. In 2005, HLB was first detected and it was estimated that 10% or 60,000 acres of citrus was destroyed by HLB by 2009, just four years after the first find. This reduction of citrus acreage in Florida corresponded with an estimated loss of 6,600 jobs, $1.3 billion in lost revenues to growers, and $3.6 billion in lost economic activity associated with the citrus industry in Florida.

The commercial citrus industry is worth about $1.2 billion in California and about 300,000 acres are farmed.

Where are ACP and HLB in the USA? ACP and HLB are present in Florida, Texas, Louisiana, Georgia, and South Carolina. ACP is present in Arizona, Mississippi, and Alabama, but HLB has not yet been found. In California, ACP was first found in San Diego and Imperial Counties in 2008. Since this initial detection ACP has been found in Ventura, Orange, Santa Barbara, Los Angeles, Riverside, and San Bernardino Counties. ACP populations at this time are heaviest in Los Angeles County.

The quarantine for HLB in Los Angeles and Orange Counties

The Response: On April 5 2012, the CDFA held an open house meeting at Industry Hills Expo Center in the City of Industry. The purpose of the meeting was to provide the public with information on HLB and ACP and to respond to requests to examine citrus with disease symptoms that look suspiciously like HLB. Additionally, all citrus within an 800 meter (about 2,400 feet) radius of the infected tree have been treated with insecticides by the CDFA to kill any Asian citrus psyllids that may be carrying bacteria that cause HLB. A 93 square mile quarantine has been established in Hacienda Heights in Los Angeles County and part of northern Orange County. This quarantine prohibits the movement of all citrus nursery stock out of this area in case they are infected with HLB and these plants, if infected, could start new disease infestations in different areas of California. Any fruit from residential properties must not be moved to other areas in case ACP or HLB accidentally hitch-hike into new areas on this fruit.

A description of the Quarantine has been prepared by CDFA and maps of the Quarantine Zone are available.

What is the Future for Citrus in California? Following the find of HLB in Hacienda Heights there has been concern that a massive citrus die off in gardens is now imminent. This is unlikely because surveys have failed so far to find more than one tree infected with HLB. Further, it is unlikely that trees dying from HLB be initially widespread, it is probable that if there are other HLB infected trees in California, they are uncommon and widespread. The difficult problem facing the CDFA and USDA is finding these infected trees (should they exist) and eradicating them before ACP finds them, feeds, acquires HLB bacteria, and spreads them to healthy citrus. Because the disease is slow in killing trees, the loss of trees will be slow, and probably patchily distributed in the early stages of the spread.

What Can you Do to Help? The CDFA has set up a Pest Hotline 1-800-491-1899 and a Report-a-Pest website http://www.cdfa.ca.gov/plant/reportapest/ to help in the fight against invasive species coming into California.

Recent News Articles
Los Angeles Times: Gardeners’ Common Bond May Have Introduced Deadly Disease. 
Los Angeles Times: San Gabriel Valley Homeowners Swarm to Meeting about Citrus Disease

Topics: Asian Citrus Psyllid, Huanglongbing, Invasive Species, Mark Hoddle, News, UC Riverside | No Comments »

Entomophagy: Collecting and Eating Red Palm Weevil Larvae from Nipa Palms in Sumatra, Indonesia

By Mark Hoddle | March 9, 2012

The red stripe form of red palm weevil (referred to here as Rhynchophorus vulneratus) is harvested for food from nipa palm trunks in parts of Sumatra by rural and semi-rural agrarians. Nipa palms (Nypah fruticans; known as buah atap in Indonesia) typically grow in swampy muddy areas (in either fresh or brackish water conditions) and the fronds are used as thatch roofing, or basket making. Most of the trunk of the nipa palm grows underground and the leaves and flower stalks grow above ground. The sap and flowers are sweet, rich in sugars, and can be fermented. The trunk is high in starch and can be harvested, pounded, and dried to form a type of sweet “sago” flour.

Another use of the palm is for the deliberate rearing of R. vulneratus larvae for harvesting and eating. Adult weevils are attracted to exposed damaged trunks and in some instances salt may be applied to the cut area which reportedly increases the attractiveness to breeding weevils. Adult weevils attracted to the palm trunk release an aggregation pheromone which further amplifies the attractiveness of the palm for breeding and feeding. Female weevils use their long rostrum or snout to chew holes into the trunk and eggs are laid (oviposited) into these holes. After several days, the eggs hatch and the small first instar weevil larvae burrow into the trunk to feed.

Palm trunks prepared for weevil attack are opened with an axe and machete after about six weeks. If the trunk is infested with weevil larvae a distinctive fermenting odor emanates from the trunk when it is opened, the internal tissue has oxidized and taken on a brownish color with interspersed ginger-colored gelatinous globs. This fed upon and fermenting material has the consistency of sloppy oatmeal (porridge). Weevil larvae feeding inside the palm trunk are easy to find because they make large diameter tunnels, and disturbance and probing of tunnels causes the legless larvae to wriggle vigorously and once they start to move they are easy to pick out of the tunnels.

The larvae are very high in protein and are considered by FAO to be an insect food that has potential to be commercialized because it requires low inputs for the quantity and quality of nutrition provided. About 1,700 species of insects are eaten by humans, mainly in the tropics because they are available year round, diversity is high, and the harvest and sale of insects for human food is a good income supplement. Human consumption of insects is called entomophagy.

Large R. vulneratus larvae harvested from nipa logs in Sumatra can sell for as much as 1000 rupiah (~ $0.11 US) each while smaller larvae fetch around 500 rupiah. Larvae can be cooked before being eaten. To prepare larvae, the large head capsule is removed, larvae may be blanched quickly in boiling water before being battered and deep fried or prepared as a type of curry. When prepared this way weevil larvae are reminiscent of cooked shrimp or squid. Another selling point for these larvae is their apparent potency as an aphrodisiac (something I can’t confirm or deny after eating many). One of our field assistants refused to eat the larvae because he was working away from his village and wouldn’t see his wife for the next two weeks. This may be a powerful testimony to the power of this aphrodisiac.

Alternatively, larvae can be eaten raw straight from the nipa trunk. Smaller larvae are preferred because they are easier to chew and swallow. However, large larvae are no problem to eat alive. The best way to eat palm weevil larvae raw is to hold them by the head capsule between your thumb and forefinger, place the larva’s body in your mouth, and bite behind the head severing it from the body and leaving the head capsule between your fingertips. Palm weevil larvae from nipa palms are surprisingly sweet, the texture internal body contents is smooth and agreeable, and they don’t have a foul or weird taste (you’d expect them to taste like rotting wood but they don’t!) The larva cuticle or skin is very chewy and can be spat out or easily swallowed.

I was very surprised at how easy these larvae were to eat straight from the nipa palm trunk in the jungle. We washed the larvae with some drinking water to remove the fermenting palm mush prior to eating them. Our guides indicated that the most preferred weevil larvae are taken from nipa palms while those harvested from the crowns of coconuts have a less agreeable taste and texture.

For more info:
Red Palm Weevil on CISR
Edible Forest Insects – Humans Bite Back!!
Beastly Bugs or Edible Delicacies?
Time for a ‘Bug Mac’? The Dutch Aim to Make Insects More Palatable
Entomophagy: Edible forest insects
Could you please pass me the bug kabobs? 

 

Topics: Entomophagy, Invasive Species, Mark Hoddle, Red Palm Weevil | 4 Comments »

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